Arabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition

Zi Wei Ye, Shiu Cheung Lung, Tai Hua Hu, Qin Fang Chen, Yung Lee Suen, Mingfu Wang, Susanne Hoffmann-Benning, Edward Yeung, Mee Len Chye*

*Corresponding author for this work

Research output: Journal PublicationsJournal Article (refereed)

17 Citations (Scopus)

Abstract

Arabidopsis thaliana ACYL-COA-BINDING PROTEIN6 (AtACBP6) encodes a cytosolic 10-kDa AtACBP. It confers freezing tolerance in transgenic Arabidopsis, possibly by its interaction with lipids as indicated by the binding of acyl-CoA esters and phosphatidylcholine to recombinant AtACBP6. Herein, transgenic Arabidopsis transformed with an AtACBP6 promoter-driven β-glucuronidase (GUS) construct exhibited strong GUS activity in the vascular tissues. Immunoelectron microscopy using anti-AtACBP6 antibodies showed AtACBP6 localization in the phloem especially in the companion cells and sieve elements. Also, the presence of gold grains in the plasmodesmata indicated its potential role in systemic trafficking. The AtACBP6 protein, but not its mRNA, was found in phloem exudate of wild-type Arabidopsis. Fatty acid profiling using gas chromatography-mass spectrometry revealed an increase in the jasmonic acid (JA) precursor, 12-oxo-cis,cis-10,15-phytodienoic acid (cis-OPDA), and a reduction in JA and/or its derivatives in acbp6 phloem exudates in comparison to the wild type. Quantitative real-time PCR showed down-regulation of COMATOSE (CTS) in acbp6 rosettes suggesting that AtACBP6 affects CTS function. AtACBP6 appeared to affect the content of JA and/or its derivatives in the sieve tubes, which is consistent with its role in pathogen-defense and in its wound-inducibility of AtACBP6pro::GUS. Taken together, our results suggest the involvement of AtACBP6 in JA-biosynthesis in Arabidopsis phloem tissues.

Original languageEnglish
Pages (from-to)717-730
Number of pages14
JournalPlant Molecular Biology
Volume92
Issue number6
DOIs
Publication statusPublished - 1 Dec 2016
Externally publishedYes

Keywords

  • Acyl-CoA-binding protein
  • Arabidopsis
  • Companion cell
  • OPDA
  • Phloem exudates
  • Sieve elements

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    Ye, Z. W., Lung, S. C., Hu, T. H., Chen, Q. F., Suen, Y. L., Wang, M., Hoffmann-Benning, S., Yeung, E., & Chye, M. L. (2016). Arabidopsis acyl-CoA-binding protein ACBP6 localizes in the phloem and affects jasmonate composition. Plant Molecular Biology, 92(6), 717-730. https://doi.org/10.1007/s11103-016-0541-0