Arabidopsis Acyl-Coenzyme-A-Binding Protein ACBP1 interacts with AREB1 and mediates salt and osmotic signaling in seed germination and seedling growth

Mo-Xian Chen, Taihua HU, Yan Xue, Fu-Yuan Zhu, Clive Lo, Mee-Len Chye

Research output: Journal PublicationsJournal Article (refereed)Researchpeer-review

1 Scopus Citations

Abstract

Arabidopsis ACYL-COENZYME-A-BINDING PROTEIN1 (ACBP1) plays a positive role in abscisic acid (ABA) signaling during seed dormancy and germination. As a putative component of the ABA signaling pathway, its potential role in salt and osmotic signaling was tested using ACBP1pro::GUS transgenic lines, ACBP1-overexpressors (ACBP1-OXs), the acbp1 mutant and ACBP1-complemented lines (acbp1-COM). ACBP1-OXs were more sensitive than the wild type to NaCl and mannitol during seed germination and seedling establishment, while the acbp1 mutant was less sensitive during seed germination. Germination assays were conducted using the ABA biosynthesis inhibitor, fluridone, to determine whether the ACBP1 response was ABA-related. The germination potential of Col-0, acbp1, ACBP1-OXs, and acbp1-COM recovered to a level similar to the water control when fluridone was applied to NaCl- and mannitol-treated seeds, indicating that phenotypic variation amongst these lines was related to de novo ABA biosynthesis. Furthermore, yeast two-hybrid, bimolecular complementation assays and pull-down assays showed that ACBP1 interacted with ABA-RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) via its ankyrin repeats. The target genes of AREB1 were up-regulated in ACBP1-OXs but down-regulated in acbp1; and the localization of DsRed-AREB1 was affected in ACBP1-OX. These results indicate that ACBP1 promotes ABA signaling under salt and osmotic stress during seed to seedling transition.
Original languageEnglish
Pages (from-to)130-140
JournalEnvironmental and Experimental Botany
Volume156
DOIs
Publication statusPublished - Dec 2018
Externally publishedYes

Fingerprint

acyl coenzyme A
abscisic acid
seedling growth
binding proteins
germination
seed germination
Arabidopsis
seedling
salt
salts
seed
protein
assay
fluridone
mannitol
seed dormancy
assays
seedling establishment
biosynthesis
ankyrins

Cite this

@article{826fa6c04d1e4d238ee097613988f1a8,
title = "Arabidopsis Acyl-Coenzyme-A-Binding Protein ACBP1 interacts with AREB1 and mediates salt and osmotic signaling in seed germination and seedling growth",
abstract = "Arabidopsis ACYL-COENZYME-A-BINDING PROTEIN1 (ACBP1) plays a positive role in abscisic acid (ABA) signaling during seed dormancy and germination. As a putative component of the ABA signaling pathway, its potential role in salt and osmotic signaling was tested using ACBP1pro::GUS transgenic lines, ACBP1-overexpressors (ACBP1-OXs), the acbp1 mutant and ACBP1-complemented lines (acbp1-COM). ACBP1-OXs were more sensitive than the wild type to NaCl and mannitol during seed germination and seedling establishment, while the acbp1 mutant was less sensitive during seed germination. Germination assays were conducted using the ABA biosynthesis inhibitor, fluridone, to determine whether the ACBP1 response was ABA-related. The germination potential of Col-0, acbp1, ACBP1-OXs, and acbp1-COM recovered to a level similar to the water control when fluridone was applied to NaCl- and mannitol-treated seeds, indicating that phenotypic variation amongst these lines was related to de novo ABA biosynthesis. Furthermore, yeast two-hybrid, bimolecular complementation assays and pull-down assays showed that ACBP1 interacted with ABA-RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) via its ankyrin repeats. The target genes of AREB1 were up-regulated in ACBP1-OXs but down-regulated in acbp1; and the localization of DsRed-AREB1 was affected in ACBP1-OX. These results indicate that ACBP1 promotes ABA signaling under salt and osmotic stress during seed to seedling transition.",
author = "Mo-Xian Chen and Taihua HU and Yan Xue and Fu-Yuan Zhu and Clive Lo and Mee-Len Chye",
year = "2018",
month = "12",
doi = "10.1016/j.envexpbot.2018.09.007",
language = "English",
volume = "156",
pages = "130--140",
journal = "Environmental and Experimental Botany",
issn = "0098-8472",
publisher = "Elsevier",

}

Arabidopsis Acyl-Coenzyme-A-Binding Protein ACBP1 interacts with AREB1 and mediates salt and osmotic signaling in seed germination and seedling growth. / Chen, Mo-Xian; HU, Taihua; Xue, Yan; Zhu, Fu-Yuan; Lo, Clive; Chye, Mee-Len.

In: Environmental and Experimental Botany, Vol. 156, 12.2018, p. 130-140.

Research output: Journal PublicationsJournal Article (refereed)Researchpeer-review

TY - JOUR

T1 - Arabidopsis Acyl-Coenzyme-A-Binding Protein ACBP1 interacts with AREB1 and mediates salt and osmotic signaling in seed germination and seedling growth

AU - Chen, Mo-Xian

AU - HU, Taihua

AU - Xue, Yan

AU - Zhu, Fu-Yuan

AU - Lo, Clive

AU - Chye, Mee-Len

PY - 2018/12

Y1 - 2018/12

N2 - Arabidopsis ACYL-COENZYME-A-BINDING PROTEIN1 (ACBP1) plays a positive role in abscisic acid (ABA) signaling during seed dormancy and germination. As a putative component of the ABA signaling pathway, its potential role in salt and osmotic signaling was tested using ACBP1pro::GUS transgenic lines, ACBP1-overexpressors (ACBP1-OXs), the acbp1 mutant and ACBP1-complemented lines (acbp1-COM). ACBP1-OXs were more sensitive than the wild type to NaCl and mannitol during seed germination and seedling establishment, while the acbp1 mutant was less sensitive during seed germination. Germination assays were conducted using the ABA biosynthesis inhibitor, fluridone, to determine whether the ACBP1 response was ABA-related. The germination potential of Col-0, acbp1, ACBP1-OXs, and acbp1-COM recovered to a level similar to the water control when fluridone was applied to NaCl- and mannitol-treated seeds, indicating that phenotypic variation amongst these lines was related to de novo ABA biosynthesis. Furthermore, yeast two-hybrid, bimolecular complementation assays and pull-down assays showed that ACBP1 interacted with ABA-RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) via its ankyrin repeats. The target genes of AREB1 were up-regulated in ACBP1-OXs but down-regulated in acbp1; and the localization of DsRed-AREB1 was affected in ACBP1-OX. These results indicate that ACBP1 promotes ABA signaling under salt and osmotic stress during seed to seedling transition.

AB - Arabidopsis ACYL-COENZYME-A-BINDING PROTEIN1 (ACBP1) plays a positive role in abscisic acid (ABA) signaling during seed dormancy and germination. As a putative component of the ABA signaling pathway, its potential role in salt and osmotic signaling was tested using ACBP1pro::GUS transgenic lines, ACBP1-overexpressors (ACBP1-OXs), the acbp1 mutant and ACBP1-complemented lines (acbp1-COM). ACBP1-OXs were more sensitive than the wild type to NaCl and mannitol during seed germination and seedling establishment, while the acbp1 mutant was less sensitive during seed germination. Germination assays were conducted using the ABA biosynthesis inhibitor, fluridone, to determine whether the ACBP1 response was ABA-related. The germination potential of Col-0, acbp1, ACBP1-OXs, and acbp1-COM recovered to a level similar to the water control when fluridone was applied to NaCl- and mannitol-treated seeds, indicating that phenotypic variation amongst these lines was related to de novo ABA biosynthesis. Furthermore, yeast two-hybrid, bimolecular complementation assays and pull-down assays showed that ACBP1 interacted with ABA-RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) via its ankyrin repeats. The target genes of AREB1 were up-regulated in ACBP1-OXs but down-regulated in acbp1; and the localization of DsRed-AREB1 was affected in ACBP1-OX. These results indicate that ACBP1 promotes ABA signaling under salt and osmotic stress during seed to seedling transition.

U2 - 10.1016/j.envexpbot.2018.09.007

DO - 10.1016/j.envexpbot.2018.09.007

M3 - Journal Article (refereed)

VL - 156

SP - 130

EP - 140

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

ER -