Chromosomal-level genome assembly and single-nucleotide polymorphism sites of black-faced spoonbill Platalea minor

Hong Kong Biodiversity Genomics Consortium, Jerome H.L. HUI*, Ting Fung CHAN, Leo Lai CHAN, Siu Gin CHEUNG, Chi Chiu CHEANG, James Kar Hei FANG, Juan Diego Gaitan-Espitia, Stanley Chun Kwan LAU, Yik Hei SUNG, Chris Kong Chu WONG, Kevin Yuk Lap YIP, Yingying WEI, Wai Lok SO, Wenyan NONG, Sean Tsz Sum LAW, Paul CROW, Aiko LEONG, Liz ROSE-JEFFREYS, Ho Yin YIP

*Corresponding author for this work

Research output: Journal PublicationsJournal Article (refereed)peer-review

Abstract

Platalea minor, or black-faced spoonbill (Threskiornithidae), is a wading bird confined to coastal areas in East Asia. Due to habitat destruction, it was classified as globally endangered by the International Union for Conservation of Nature. However, the lack of genomic resources for this species hinders the understanding of its biology and diversity, and the development of conservation measures. Here, we report the first chromosomal-level genome assembly of P. minor using a combination of PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (1.24 Gb) contains 95.33% of the sequences anchored to 31 pseudomolecules. The genome assembly has high sequence continuity with scaffold length N50 = 53 Mb. We predicted 18,780 protein-coding genes and measured high BUSCO score completeness (97.3%). Finally, we revealed 6,155,417 bi-allelic single nucleotide polymorphisms, accounting for ∼5% of the genome. This resource offers new opportunities for studying the black-faced spoonbill and developing conservation measures for this species.

Original languageEnglish
Number of pages13
JournalGigaByte
Volume2024
DOIs
Publication statusE-pub ahead of print - 18 Jul 2024

Bibliographical note

Publisher Copyright:
© 2024 GigaScience Press. All rights reserved.

Funding

This work was funded and supported by the Hong Kong Research Grant Council Collaborative Research Fund (C4015-20EF), CUHK Strategic Seed Funding for Collaborative Research Scheme (3133356) and CUHK Group Research Scheme (3110154).

Keywords

  • Animal Genetics
  • Ecology
  • Genetics and Genomics

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