Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860)

Hong Kong Biodiversity Genomics Consortium; Jerome H.L. HUI; Ting Fung CHAN; Leo Lai CHAN; Siu Gin CHEUNG; Chi Chiu CHEANG; James Kar Hei Fang; Juan Diego GAITAN-ESPITIA; Stanley Chun Kwan LAU; Yik Hei SUNG; Chris Kong Chu WONG; Kevin Yuk Lap YIP; Yingying WEI; Wai Lok SO; Wenyan NONG; Hydrogen Sui Fai PUN; Wing Kwong YAU; Colleen Yuk Lin CHIU; Sammi Shan Shan CHAN; Kacy Ka Ling MAN; Ho Yin YIP

doi:10.46471/gigabyte.122

Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860)

Hong Kong Biodiversity Genomics Consortium, Jerome H.L. HUI^*, Ting Fung CHAN, Leo Lai CHAN, Siu Gin CHEUNG, Chi Chiu CHEANG, James Kar Hei Fang, Juan Diego GAITAN-ESPITIA, Stanley Chun Kwan LAU, Yik Hei SUNG, Chris Kong Chu WONG, Kevin Yuk Lap YIP, Yingying WEI, Wai Lok SO, Wenyan NONG, Hydrogen Sui Fai PUN, Wing Kwong YAU, Colleen Yuk Lin CHIU, Sammi Shan Shan CHAN, Kacy Ka Ling MANHo Yin YIP

^*Corresponding author for this work

Science Unit

Research output: Journal Publications › Journal Article (refereed) › peer-review

Abstract

The golden birdwing Troides aeacus (Lepidoptera, Papilionidae), a significant species in Asia, faces habitat loss due to urbanization and human activities, necessitating its protection. However, the lack of genomic resources hinders our understanding of their biology and diversity, and impedes our conservation efforts based on genetic information or markers. Here, we present the first chromosomal-level genome assembly of T. aeacus using PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (351 Mb) contains 98.94% of the sequences anchored to 30 pseudo-molecules. The genome assembly has high sequence continuity with contig length N50 = 11.67 Mb and L50 = 14, and scaffold length N50 = 12.2 Mb and L50 = 13. A total of 24,946 protein-coding genes were predicted, with high BUSCO score completeness (98.8% and 94.7% of genome and proteome BUSCO, respectively. This genome offers a significant resource for understanding the swallowtail butterfly biology and carrying out its conservation.

Original language	English
Journal	GigaByte
Volume	2024
DOIs	https://doi.org/10.46471/gigabyte.122
Publication status	Published - 2024

Bibliographical note

Publisher Copyright:
© 2024 GigaScience Press. All rights reserved.

Funding

This work was funded and supported by the Hong Kong Research Grant Council Collaborative Research Fund (C4015-20EF), CUHK Strategic Seed Funding for Collaborative Research Scheme (3133356), and CUHK Group Research Scheme (3110154).

Keywords

Animal Genetics
Biodiversity
Genetics and Genomics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.46471/gigabyte.122

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Hong Kong Biodiversity Genomics Consortium, HUI, J. H. L., CHAN, T. F., CHAN, L. L., CHEUNG, S. G., CHEANG, C. C., Fang, J. K. H., GAITAN-ESPITIA, J. D., LAU, S. C. K., SUNG, Y. H., WONG, C. K. C., YIP, K. Y. L., WEI, Y., SO, W. L., NONG, W., PUN, H. S. F., YAU, W. K., CHIU, C. Y. L., CHAN, S. S. S., ... YIP, H. Y. (2024). Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860). GigaByte, 2024. https://doi.org/10.46471/gigabyte.122

@article{d49963ebdbeb422da473cd3df32c8845,

title = "Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860)",

abstract = "The golden birdwing Troides aeacus (Lepidoptera, Papilionidae), a significant species in Asia, faces habitat loss due to urbanization and human activities, necessitating its protection. However, the lack of genomic resources hinders our understanding of their biology and diversity, and impedes our conservation efforts based on genetic information or markers. Here, we present the first chromosomal-level genome assembly of T. aeacus using PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (351 Mb) contains 98.94% of the sequences anchored to 30 pseudo-molecules. The genome assembly has high sequence continuity with contig length N50 = 11.67 Mb and L50 = 14, and scaffold length N50 = 12.2 Mb and L50 = 13. A total of 24,946 protein-coding genes were predicted, with high BUSCO score completeness (98.8% and 94.7% of genome and proteome BUSCO, respectively. This genome offers a significant resource for understanding the swallowtail butterfly biology and carrying out its conservation.",

keywords = "Animal Genetics, Biodiversity, Genetics and Genomics",

author = "{Hong Kong Biodiversity Genomics Consortium} and HUI, {Jerome H.L.} and CHAN, {Ting Fung} and CHAN, {Leo Lai} and CHEUNG, {Siu Gin} and CHEANG, {Chi Chiu} and Fang, {James Kar Hei} and GAITAN-ESPITIA, {Juan Diego} and LAU, {Stanley Chun Kwan} and SUNG, {Yik Hei} and WONG, {Chris Kong Chu} and YIP, {Kevin Yuk Lap} and Yingying WEI and SO, {Wai Lok} and Wenyan NONG and PUN, {Hydrogen Sui Fai} and YAU, {Wing Kwong} and CHIU, {Colleen Yuk Lin} and CHAN, {Sammi Shan Shan} and MAN, {Kacy Ka Ling} and YIP, {Ho Yin}",

year = "2024",

doi = "10.46471/gigabyte.122",

language = "English",

volume = "2024",

journal = "GigaByte",

issn = "2709-4715",

publisher = "GigaScience Press",

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Hong Kong Biodiversity Genomics Consortium, HUI, JHL, CHAN, TF, CHAN, LL, CHEUNG, SG, CHEANG, CC, Fang, JKH, GAITAN-ESPITIA, JD, LAU, SCK, SUNG, YH, WONG, CKC, YIP, KYL, WEI, Y, SO, WL, NONG, W, PUN, HSF, YAU, WK, CHIU, CYL, CHAN, SSS, MAN, KKL & YIP, HY 2024, 'Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860)', GigaByte, vol. 2024. https://doi.org/10.46471/gigabyte.122

TY - JOUR

T1 - Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860)

AU - Hong Kong Biodiversity Genomics Consortium

AU - HUI, Jerome H.L.

AU - CHAN, Ting Fung

AU - CHAN, Leo Lai

AU - CHEUNG, Siu Gin

AU - CHEANG, Chi Chiu

AU - Fang, James Kar Hei

AU - GAITAN-ESPITIA, Juan Diego

AU - LAU, Stanley Chun Kwan

AU - SUNG, Yik Hei

AU - WONG, Chris Kong Chu

AU - YIP, Kevin Yuk Lap

AU - WEI, Yingying

AU - SO, Wai Lok

AU - NONG, Wenyan

AU - PUN, Hydrogen Sui Fai

AU - YAU, Wing Kwong

AU - CHIU, Colleen Yuk Lin

AU - CHAN, Sammi Shan Shan

AU - MAN, Kacy Ka Ling

AU - YIP, Ho Yin

PY - 2024

Y1 - 2024

N2 - The golden birdwing Troides aeacus (Lepidoptera, Papilionidae), a significant species in Asia, faces habitat loss due to urbanization and human activities, necessitating its protection. However, the lack of genomic resources hinders our understanding of their biology and diversity, and impedes our conservation efforts based on genetic information or markers. Here, we present the first chromosomal-level genome assembly of T. aeacus using PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (351 Mb) contains 98.94% of the sequences anchored to 30 pseudo-molecules. The genome assembly has high sequence continuity with contig length N50 = 11.67 Mb and L50 = 14, and scaffold length N50 = 12.2 Mb and L50 = 13. A total of 24,946 protein-coding genes were predicted, with high BUSCO score completeness (98.8% and 94.7% of genome and proteome BUSCO, respectively. This genome offers a significant resource for understanding the swallowtail butterfly biology and carrying out its conservation.

AB - The golden birdwing Troides aeacus (Lepidoptera, Papilionidae), a significant species in Asia, faces habitat loss due to urbanization and human activities, necessitating its protection. However, the lack of genomic resources hinders our understanding of their biology and diversity, and impedes our conservation efforts based on genetic information or markers. Here, we present the first chromosomal-level genome assembly of T. aeacus using PacBio SMRT and Omni-C scaffolding technologies. The assembled genome (351 Mb) contains 98.94% of the sequences anchored to 30 pseudo-molecules. The genome assembly has high sequence continuity with contig length N50 = 11.67 Mb and L50 = 14, and scaffold length N50 = 12.2 Mb and L50 = 13. A total of 24,946 protein-coding genes were predicted, with high BUSCO score completeness (98.8% and 94.7% of genome and proteome BUSCO, respectively. This genome offers a significant resource for understanding the swallowtail butterfly biology and carrying out its conservation.

KW - Animal Genetics

KW - Biodiversity

KW - Genetics and Genomics

UR - http://www.scopus.com/inward/record.url?scp=85193733839&partnerID=8YFLogxK

U2 - 10.46471/gigabyte.122

DO - 10.46471/gigabyte.122

M3 - Journal Article (refereed)

C2 - 38707630

AN - SCOPUS:85193733839

SN - 2709-4715

VL - 2024

JO - GigaByte

JF - GigaByte

ER -

Chromosomal-level genome assembly of golden birdwing Troides aeacus (Felder & Felder, 1860)

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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