Delimitation of Russula subgenus Amoenula in Korea using three molecular markers

Myung Soo PARK, Jonathan J. FONG, Hyun LEE, Seung-Yoon OH, Paul Eunil JUNG, Young Ju MIN, Soon Ja SEOK, Young Woon LIM

Research output: Journal PublicationsJournal Article (refereed)Researchpeer-review

26 Citations (Scopus)

Abstract

Distinguishing individual Russula species has been difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. Due to highly similar macroscopic features, such as the presence of a red-cap, species identification within the Russula subgenus Amoenula is particularly difficult. Three species of the subgenus Amoneula have been reported in Korea. We used a combination of morphology and three molecular markers, the internal transcribed spacer (ITS), 28S nuclear ribosomal large subunit (LSU), and RNA polymerase II gene (RPB2), for identification and study of the genetic diversity of Russula subgenus Amoenula in Korea. We identified only two species in Korea ( R. mariae and R. violeipes ); these two species were indistinguishable according to morphology and LSU, but were found to be reciprocally monophyletic species using ITS and RPB2. The markers, ITS, LSU, and RPB2, have been tested in the past for use as DNA barcoding markers, and findings of our study suggest that ITS and RPB2 had the best performance for the Russula subgenus Amoneula .
Original languageEnglish
Pages (from-to)191-201
Number of pages11
JournalMycobiology
Volume41
Issue number4
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

Fingerprint

Korea
Nuclear RNA
Ribosome Subunits
Ribosomal RNA
RNA Polymerase II
Genetic Markers
Genes

Keywords

  • DNA barcoding
  • Internal transcribed spacer
  • RNA polymerase II gene
  • Russula mariae
  • Russula violeipes
  • 28S nuclear ribosomal large subunit

Cite this

PARK, M. S., FONG, J. J., LEE, H., OH, S-Y., JUNG, P. E., MIN, Y. J., ... LIM, Y. W. (2013). Delimitation of Russula subgenus Amoenula in Korea using three molecular markers. Mycobiology, 41(4), 191-201. https://doi.org/10.5941/MYCO.2013.41.4.191
PARK, Myung Soo ; FONG, Jonathan J. ; LEE, Hyun ; OH, Seung-Yoon ; JUNG, Paul Eunil ; MIN, Young Ju ; SEOK, Soon Ja ; LIM, Young Woon. / Delimitation of Russula subgenus Amoenula in Korea using three molecular markers. In: Mycobiology. 2013 ; Vol. 41, No. 4. pp. 191-201.
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abstract = "Distinguishing individual Russula species has been difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. Due to highly similar macroscopic features, such as the presence of a red-cap, species identification within the Russula subgenus Amoenula is particularly difficult. Three species of the subgenus Amoneula have been reported in Korea. We used a combination of morphology and three molecular markers, the internal transcribed spacer (ITS), 28S nuclear ribosomal large subunit (LSU), and RNA polymerase II gene (RPB2), for identification and study of the genetic diversity of Russula subgenus Amoenula in Korea. We identified only two species in Korea ( R. mariae and R. violeipes ); these two species were indistinguishable according to morphology and LSU, but were found to be reciprocally monophyletic species using ITS and RPB2. The markers, ITS, LSU, and RPB2, have been tested in the past for use as DNA barcoding markers, and findings of our study suggest that ITS and RPB2 had the best performance for the Russula subgenus Amoneula .",
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PARK, MS, FONG, JJ, LEE, H, OH, S-Y, JUNG, PE, MIN, YJ, SEOK, SJ & LIM, YW 2013, 'Delimitation of Russula subgenus Amoenula in Korea using three molecular markers', Mycobiology, vol. 41, no. 4, pp. 191-201. https://doi.org/10.5941/MYCO.2013.41.4.191

Delimitation of Russula subgenus Amoenula in Korea using three molecular markers. / PARK, Myung Soo; FONG, Jonathan J.; LEE, Hyun; OH, Seung-Yoon; JUNG, Paul Eunil; MIN, Young Ju; SEOK, Soon Ja; LIM, Young Woon.

In: Mycobiology, Vol. 41, No. 4, 12.2013, p. 191-201.

Research output: Journal PublicationsJournal Article (refereed)Researchpeer-review

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AU - SEOK, Soon Ja

AU - LIM, Young Woon

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AB - Distinguishing individual Russula species has been difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. Due to highly similar macroscopic features, such as the presence of a red-cap, species identification within the Russula subgenus Amoenula is particularly difficult. Three species of the subgenus Amoneula have been reported in Korea. We used a combination of morphology and three molecular markers, the internal transcribed spacer (ITS), 28S nuclear ribosomal large subunit (LSU), and RNA polymerase II gene (RPB2), for identification and study of the genetic diversity of Russula subgenus Amoenula in Korea. We identified only two species in Korea ( R. mariae and R. violeipes ); these two species were indistinguishable according to morphology and LSU, but were found to be reciprocally monophyletic species using ITS and RPB2. The markers, ITS, LSU, and RPB2, have been tested in the past for use as DNA barcoding markers, and findings of our study suggest that ITS and RPB2 had the best performance for the Russula subgenus Amoneula .

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