Diversity and saline resistance of endophytic fungi associated with pinus thunbergii in coastal shelterbelts of Korea

Young Ju MIN, Myung Soo PARK, Jonathan, J. FONG, Ying QUAN, Sungcheol JUNG, Young Woon LIM

Research output: Journal PublicationsJournal Article (refereed)

15 Citations (Scopus)


The Black Pine, Pinus thunbergii, is widely distributed along the eastern coast of Korea and its importance as a shelterbelt was highlighted after tsunamis in Indonesia and Japan. The root endophytic diversity of P. thunbergii was investigated in three coastal regions; Goseong, Uljin, and Busan. Fungi were isolated from the root tips, and growth rates of pure cultures were measured and compared between PDA with and without 3% NaCl to determine their saline resistance. A total of 259 isolates were divided into 136 morphotypes, of which internal transcribed spacer region sequences identified 58 species. Representatives of each major fungi phylum were present: 44 Ascomycota, 8 Zygomycota, and 6 Basidiomycota. Eighteen species exhibited saline resistance, many of which were Penicillium and Trichoderma species. Shoreline habitats harbored higher saline-tolerant endophytic diversity compared with inland sites. This investigation indicates that endophytes of P. thunbergii living closer to the coast may have higher resistance to salinity and potentially have specific relationships with P. thunbergii.
Original languageEnglish
Pages (from-to)324-333
Number of pages10
JournalJournal of Microbiology and Biotechnology
Issue number3
Early online date9 Dec 2013
Publication statusPublished - Mar 2014
Externally publishedYes



  • Coastal pine forest
  • endophyte community
  • salinity tolerance
  • shelterbelt

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