Evaluating noninvasive methods of sex identification in Green Sea Turtle (Chelonia mydas) hatchlings

Zhong Rong XIA, Pi Peng LI, He Xiang GU, Jonathan J. FONG, Er Mi ZHAO

Research output: Journal PublicationsJournal Article (refereed)

12 Citations (Scopus)


The goal of this study was to test the effectiveness of analyzing sex hormone profiles to sex green sea turtle ( Chelonia mydas ) hatchlings. To assess the efficacy of this method, 200 eggs of C. mydas were incubated at five different temperatures, and a chemiluminescent immunoassay system was used to determine the sex hormone profiles (estrogen and testosterone) in the amniotic fluid (from eggshells) and plasma of a subset of C. mydas hatchlings. Results were compared to a standard histological technique and revealed that evaluating sex hormone profiles from amniotic fluid is an effective, noninvasive technique for determining hatchling gender.
Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalChelonian Conservation and Biology
Issue number1
Publication statusPublished - 1 Jul 2011
Externally publishedYes


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