Abstract
The goal of this study was to test the effectiveness of analyzing sex hormone profiles to sex green sea turtle ( Chelonia mydas ) hatchlings. To assess the efficacy of this method, 200 eggs of C. mydas were incubated at five different temperatures, and a chemiluminescent immunoassay system was used to determine the sex hormone profiles (estrogen and testosterone) in the amniotic fluid (from eggshells) and plasma of a subset of C. mydas hatchlings. Results were compared to a standard histological technique and revealed that evaluating sex hormone profiles from amniotic fluid is an effective, noninvasive technique for determining hatchling gender.
| Original language | English |
|---|---|
| Pages (from-to) | 117-123 |
| Number of pages | 7 |
| Journal | Chelonian Conservation and Biology |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jul 2011 |
| Externally published | Yes |
Bibliographical note
Our work is permitted by Huidong Gangkou Sea Turtle National Nature Reserve. We greatly appreciate the assistance of staff from the HGSTNNR.Funding
This research was funded by the grants from the Science and Technology Department of Guangdong Province (the project numbers: 2006B60101011) and the Marine Fisheries Science and Technology special promote and major projects of Guangdong Province (B200613G11; A2009010-006; A2010006-003).