Identifying airborne fungi in Seoul, Korea using metagenomics

Seung-Yoon OH, Jonathan, Julio FONG, Myung Soo PARK, Limseok CHANG, Young Woon LIM

Research output: Journal PublicationsJournal Article (refereed)

25 Citations (Scopus)

Abstract

Fungal spores are widespread and common in the atmosphere. In this study, we use a metagenomic approach to study the fungal diversity in six total air samples collected from April to May 2012 in Seoul, Korea. This springtime period is important in Korea because of the peak in fungal spore concentration and Asian dust storms, although the year of this study (2012) was unique in that were no major Asian dust events. Clustering sequences for operational taxonomic unit (OTU) identification recovered 1,266 unique OTUs in the combined dataset, with between 223᾿96 OTUs present in individual samples. OTUs from three fungal phyla were identified. For Ascomycota, Davidiella (anamorph: Cladosporium ) was the most common genus in all samples, often accounting for more than 50% of all sequences in a sample. Other common Ascomycota genera identified were Alternaria, Didymella, Khuskia, Geosmitha, Penicillium , and Aspergillus . While several Basidiomycota genera were observed, Chytridiomycota OTUs were only present in one sample. Consistency was observed within sampling days, but there was a large shift in species composition from Ascomycota dominant to Basidiomycota dominant in the middle of the sampling period. This marked change may have been caused by meteorological events. A potential set of 40 allergyinducing genera were identified, accounting for a large proportion of the diversity present (22.5᾿7.2%). Our study identifies high fungal diversity and potentially high levels of fungal allergens in springtime air of Korea, and provides a good baseline for future comparisons with Asian dust storms.
Original languageEnglish
Pages (from-to)465-472
Number of pages8
JournalJournal of Microbiology
Volume52
Issue number6
Early online date11 Apr 2014
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

Fingerprint

Metagenomics
Ascomycota
Korea
Dust
Basidiomycota
Fungi
Fungal Spores
Chytridiomycota
Air
Cladosporium
Alternaria
Penicillium
Aspergillus
Atmosphere
Allergens
Cluster Analysis
Seoul

Keywords

  • allergen
  • fungi
  • metagenomics
  • springtime

Cite this

OH, Seung-Yoon ; FONG, Jonathan, Julio ; PARK, Myung Soo ; CHANG, Limseok ; LIM, Young Woon. / Identifying airborne fungi in Seoul, Korea using metagenomics. In: Journal of Microbiology. 2014 ; Vol. 52, No. 6. pp. 465-472.
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abstract = "Fungal spores are widespread and common in the atmosphere. In this study, we use a metagenomic approach to study the fungal diversity in six total air samples collected from April to May 2012 in Seoul, Korea. This springtime period is important in Korea because of the peak in fungal spore concentration and Asian dust storms, although the year of this study (2012) was unique in that were no major Asian dust events. Clustering sequences for operational taxonomic unit (OTU) identification recovered 1,266 unique OTUs in the combined dataset, with between 223᾿96 OTUs present in individual samples. OTUs from three fungal phyla were identified. For Ascomycota, Davidiella (anamorph: Cladosporium ) was the most common genus in all samples, often accounting for more than 50{\%} of all sequences in a sample. Other common Ascomycota genera identified were Alternaria, Didymella, Khuskia, Geosmitha, Penicillium , and Aspergillus . While several Basidiomycota genera were observed, Chytridiomycota OTUs were only present in one sample. Consistency was observed within sampling days, but there was a large shift in species composition from Ascomycota dominant to Basidiomycota dominant in the middle of the sampling period. This marked change may have been caused by meteorological events. A potential set of 40 allergyinducing genera were identified, accounting for a large proportion of the diversity present (22.5᾿7.2{\%}). Our study identifies high fungal diversity and potentially high levels of fungal allergens in springtime air of Korea, and provides a good baseline for future comparisons with Asian dust storms.",
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Identifying airborne fungi in Seoul, Korea using metagenomics. / OH, Seung-Yoon; FONG, Jonathan, Julio; PARK, Myung Soo; CHANG, Limseok; LIM, Young Woon.

In: Journal of Microbiology, Vol. 52, No. 6, 06.2014, p. 465-472.

Research output: Journal PublicationsJournal Article (refereed)

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N2 - Fungal spores are widespread and common in the atmosphere. In this study, we use a metagenomic approach to study the fungal diversity in six total air samples collected from April to May 2012 in Seoul, Korea. This springtime period is important in Korea because of the peak in fungal spore concentration and Asian dust storms, although the year of this study (2012) was unique in that were no major Asian dust events. Clustering sequences for operational taxonomic unit (OTU) identification recovered 1,266 unique OTUs in the combined dataset, with between 223᾿96 OTUs present in individual samples. OTUs from three fungal phyla were identified. For Ascomycota, Davidiella (anamorph: Cladosporium ) was the most common genus in all samples, often accounting for more than 50% of all sequences in a sample. Other common Ascomycota genera identified were Alternaria, Didymella, Khuskia, Geosmitha, Penicillium , and Aspergillus . While several Basidiomycota genera were observed, Chytridiomycota OTUs were only present in one sample. Consistency was observed within sampling days, but there was a large shift in species composition from Ascomycota dominant to Basidiomycota dominant in the middle of the sampling period. This marked change may have been caused by meteorological events. A potential set of 40 allergyinducing genera were identified, accounting for a large proportion of the diversity present (22.5᾿7.2%). Our study identifies high fungal diversity and potentially high levels of fungal allergens in springtime air of Korea, and provides a good baseline for future comparisons with Asian dust storms.

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