Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong

Priscilla T.Y. LEUNG; Meng YAN; Sam K.F. YIU; Veronica T.T. LAM; Jack C.H. IP; Maggie W.Y. AU; Chia-Yun CHEN; Tak-Cheung WAI; Paul K.S. LAM

doi:10.1016/j.marpolbul.2017.01.017

Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong

Priscilla T.Y. LEUNG, Meng YAN, Sam K.F. YIU, Veronica T.T. LAM, Jack C.H. IP, Maggie W.Y. AU, Chia-Yun CHEN, Tak-Cheung WAI^*, Paul K.S. LAM

^*Corresponding author for this work

Research output: Journal Publications › Journal Article (refereed) › peer-review

27 Citations (Scopus)

Abstract

Coolia are marine benthic dinoflagellates which are globally distributed and potentially toxic. This study provides the first investigation of species diversity and toxicity assessment of Coolia in Hong Kong waters. Fifty-one strains of four Coolia species, including C. malayensis, C. canariensis, C. tropicalis, and C. palmyrensis, were isolated from twelve sub-tidal habitats, and identified phylogenetically using 28S rDNA sequences. Exposure experiments (48-hour) demonstrated that the algal lysates extracted from the four Coolia species exhibited different toxic effects on the lethality and abnormality of two invertebrate larvae, i.e., brine shrimp Artemia franciscana and sea urchin Heliocidaris crassispina. Heliocidaris crassispina was more sensitive to the toxic effects of Coolia species than A. franciscana. Toxicity tests from both larvae revealed that C. malayensis was generally more toxic, and caused higher mortality rates when compared with the other three species. The emerging threat of harmful benthic dinoflagellates to marine environments and sensitive biota is discussed.

Original language	English
Pages (from-to)	878-889
Number of pages	12
Journal	Marine Pollution Bulletin
Volume	124
Issue number	2
Early online date	27 Jan 2017
DOIs	https://doi.org/10.1016/j.marpolbul.2017.01.017
Publication status	Published - 30 Nov 2017
Externally published	Yes

Bibliographical note

Funding Information:
This project was conducted at the State Key Laboratory in Marine Pollution and supported by the following funding sources: Collaborative Research Fund (C1012-15G to PKS Lam); Environment and Conservation Fund (Project 12/2012 to TC Wai); National Natural Science Foundation of China (41576113 to PTY Leung; and 41406184 to Meng YAN) and 2014 GD-HK TCFS Program via Innovation and Technology Commission of the Hong Kong SAR Government (GHP/016/14SZ to PTY Leung). The authors thank Zoe Ho, Teresa Ma, Jiarui Gu, Roy Lam and Michael Chiang (BCH, CityU) for their technique support. The authors also thank the anonymous reviewers for their useful comments and suggestions on the manuscript.

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

Benthic dinoflagellate
Coolia
Gambierdiscus
Invertebrate bioassay
Sea urchin
Toxic algae

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.marpolbul.2017.01.017

Cite this

LEUNG, P. T. Y., YAN, M., YIU, S. K. F., LAM, V. T. T., IP, J. C. H., AU, M. W. Y., CHEN, C.-Y., WAI, T.-C., & LAM, P. K. S. (2017). Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong. Marine Pollution Bulletin, 124(2), 878-889. https://doi.org/10.1016/j.marpolbul.2017.01.017

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title = "Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong",

abstract = "Coolia are marine benthic dinoflagellates which are globally distributed and potentially toxic. This study provides the first investigation of species diversity and toxicity assessment of Coolia in Hong Kong waters. Fifty-one strains of four Coolia species, including C. malayensis, C. canariensis, C. tropicalis, and C. palmyrensis, were isolated from twelve sub-tidal habitats, and identified phylogenetically using 28S rDNA sequences. Exposure experiments (48-hour) demonstrated that the algal lysates extracted from the four Coolia species exhibited different toxic effects on the lethality and abnormality of two invertebrate larvae, i.e., brine shrimp Artemia franciscana and sea urchin Heliocidaris crassispina. Heliocidaris crassispina was more sensitive to the toxic effects of Coolia species than A. franciscana. Toxicity tests from both larvae revealed that C. malayensis was generally more toxic, and caused higher mortality rates when compared with the other three species. The emerging threat of harmful benthic dinoflagellates to marine environments and sensitive biota is discussed.",

keywords = "Benthic dinoflagellate, Coolia, Gambierdiscus, Invertebrate bioassay, Sea urchin, Toxic algae",

author = "LEUNG, {Priscilla T.Y.} and Meng YAN and YIU, {Sam K.F.} and LAM, {Veronica T.T.} and IP, {Jack C.H.} and AU, {Maggie W.Y.} and Chia-Yun CHEN and Tak-Cheung WAI and LAM, {Paul K.S.}",

note = "Funding Information: This project was conducted at the State Key Laboratory in Marine Pollution and supported by the following funding sources: Collaborative Research Fund (C1012-15G to PKS Lam); Environment and Conservation Fund (Project 12/2012 to TC Wai); National Natural Science Foundation of China (41576113 to PTY Leung; and 41406184 to Meng YAN) and 2014 GD-HK TCFS Program via Innovation and Technology Commission of the Hong Kong SAR Government (GHP/016/14SZ to PTY Leung). The authors thank Zoe Ho, Teresa Ma, Jiarui Gu, Roy Lam and Michael Chiang (BCH, CityU) for their technique support. The authors also thank the anonymous reviewers for their useful comments and suggestions on the manuscript. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

month = nov,

day = "30",

doi = "10.1016/j.marpolbul.2017.01.017",

language = "English",

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LEUNG, PTY, YAN, M, YIU, SKF, LAM, VTT, IP, JCH, AU, MWY, CHEN, C-Y, WAI, T-C & LAM, PKS 2017, 'Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong', Marine Pollution Bulletin, vol. 124, no. 2, pp. 878-889. https://doi.org/10.1016/j.marpolbul.2017.01.017

Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong. / LEUNG, Priscilla T.Y.; YAN, Meng; YIU, Sam K.F. et al.
In: Marine Pollution Bulletin, Vol. 124, No. 2, 30.11.2017, p. 878-889.

Research output: Journal Publications › Journal Article (refereed) › peer-review

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T1 - Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong

AU - LEUNG, Priscilla T.Y.

AU - YAN, Meng

AU - YIU, Sam K.F.

AU - LAM, Veronica T.T.

AU - IP, Jack C.H.

AU - AU, Maggie W.Y.

AU - CHEN, Chia-Yun

AU - WAI, Tak-Cheung

AU - LAM, Paul K.S.

N1 - Funding Information: This project was conducted at the State Key Laboratory in Marine Pollution and supported by the following funding sources: Collaborative Research Fund (C1012-15G to PKS Lam); Environment and Conservation Fund (Project 12/2012 to TC Wai); National Natural Science Foundation of China (41576113 to PTY Leung; and 41406184 to Meng YAN) and 2014 GD-HK TCFS Program via Innovation and Technology Commission of the Hong Kong SAR Government (GHP/016/14SZ to PTY Leung). The authors thank Zoe Ho, Teresa Ma, Jiarui Gu, Roy Lam and Michael Chiang (BCH, CityU) for their technique support. The authors also thank the anonymous reviewers for their useful comments and suggestions on the manuscript. Publisher Copyright: © 2017 Elsevier Ltd

PY - 2017/11/30

Y1 - 2017/11/30

N2 - Coolia are marine benthic dinoflagellates which are globally distributed and potentially toxic. This study provides the first investigation of species diversity and toxicity assessment of Coolia in Hong Kong waters. Fifty-one strains of four Coolia species, including C. malayensis, C. canariensis, C. tropicalis, and C. palmyrensis, were isolated from twelve sub-tidal habitats, and identified phylogenetically using 28S rDNA sequences. Exposure experiments (48-hour) demonstrated that the algal lysates extracted from the four Coolia species exhibited different toxic effects on the lethality and abnormality of two invertebrate larvae, i.e., brine shrimp Artemia franciscana and sea urchin Heliocidaris crassispina. Heliocidaris crassispina was more sensitive to the toxic effects of Coolia species than A. franciscana. Toxicity tests from both larvae revealed that C. malayensis was generally more toxic, and caused higher mortality rates when compared with the other three species. The emerging threat of harmful benthic dinoflagellates to marine environments and sensitive biota is discussed.

AB - Coolia are marine benthic dinoflagellates which are globally distributed and potentially toxic. This study provides the first investigation of species diversity and toxicity assessment of Coolia in Hong Kong waters. Fifty-one strains of four Coolia species, including C. malayensis, C. canariensis, C. tropicalis, and C. palmyrensis, were isolated from twelve sub-tidal habitats, and identified phylogenetically using 28S rDNA sequences. Exposure experiments (48-hour) demonstrated that the algal lysates extracted from the four Coolia species exhibited different toxic effects on the lethality and abnormality of two invertebrate larvae, i.e., brine shrimp Artemia franciscana and sea urchin Heliocidaris crassispina. Heliocidaris crassispina was more sensitive to the toxic effects of Coolia species than A. franciscana. Toxicity tests from both larvae revealed that C. malayensis was generally more toxic, and caused higher mortality rates when compared with the other three species. The emerging threat of harmful benthic dinoflagellates to marine environments and sensitive biota is discussed.

KW - Benthic dinoflagellate

KW - Coolia

KW - Gambierdiscus

KW - Invertebrate bioassay

KW - Sea urchin

KW - Toxic algae

UR - http://www.scopus.com/inward/record.url?scp=85010916690&partnerID=8YFLogxK

U2 - 10.1016/j.marpolbul.2017.01.017

DO - 10.1016/j.marpolbul.2017.01.017

M3 - Journal Article (refereed)

C2 - 28139234

AN - SCOPUS:85010916690

SN - 0025-326X

VL - 124

SP - 878

EP - 889

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

IS - 2

ER -

Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia (Ostreopsidaceae, Dinophyceae) in a sub-tropical marine ecosystem : The first record from Hong Kong

Abstract

Bibliographical note

Keywords

UN SDGs

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