Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure

Priscilla T.Y. LEUNG; Andy Xianliang YI; Jack C.H. IP; Sarah S.T. MAK; Kenneth M.Y. LEUNG

doi:10.1016/j.marpolbul.2017.03.038

Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure

Priscilla T.Y. LEUNG^*, Andy Xianliang YI, Jack C.H. IP, Sarah S.T. MAK, Kenneth M.Y. LEUNG

^*Corresponding author for this work

Research output: Journal Publications › Journal Article (refereed) › peer-review

29 Citations (Scopus)

Abstract

A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200 μg/L Cu, EC₅₀ for the yield at 25 °C; and 1000 μg/L Cu, EC₅₀ for growth inhibition at 25 °C) were conducted against four temperatures (10 °C, 15 °C, 25 °C and 30 °C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30 °C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90–2 and sit1 (related to silica shell formation) were highly expressed at 30 °C under 1000 μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15 °C under 200 μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.

Original language	English
Pages (from-to)	938-945
Number of pages	8
Journal	Marine Pollution Bulletin
Volume	124
Issue number	2
Early online date	30 Mar 2017
DOIs	https://doi.org/10.1016/j.marpolbul.2017.03.038
Publication status	Published - 30 Nov 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Ltd

Funding

This work is supported by a General Research Fund of Research Grants Council (Project number: 17305715 ) awarded to KMY Leung and two awards of the Small Project Fund from the University of Hong Kong to KMY Leung and PTY Leung, respectively. The authors thank Dr. Edward Lau for proofreading this manuscript.

Keywords

Copper
Heat shock protein
Marine diatom
Photosynthesis
Thalassiosira
Thermal stress

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.marpolbul.2017.03.038

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title = "Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure",

abstract = "A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200 μg/L Cu, EC50 for the yield at 25 °C; and 1000 μg/L Cu, EC50 for growth inhibition at 25 °C) were conducted against four temperatures (10 °C, 15 °C, 25 °C and 30 °C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30 °C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90–2 and sit1 (related to silica shell formation) were highly expressed at 30 °C under 1000 μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15 °C under 200 μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.",

keywords = "Copper, Heat shock protein, Marine diatom, Photosynthesis, Thalassiosira, Thermal stress",

author = "LEUNG, {Priscilla T.Y.} and YI, {Andy Xianliang} and IP, {Jack C.H.} and MAK, {Sarah S.T.} and LEUNG, {Kenneth M.Y.}",

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doi = "10.1016/j.marpolbul.2017.03.038",

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Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure. / LEUNG, Priscilla T.Y.; YI, Andy Xianliang; IP, Jack C.H. et al.
In: Marine Pollution Bulletin, Vol. 124, No. 2, 30.11.2017, p. 938-945.

Research output: Journal Publications › Journal Article (refereed) › peer-review

TY - JOUR

T1 - Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure

AU - LEUNG, Priscilla T.Y.

AU - YI, Andy Xianliang

AU - IP, Jack C.H.

AU - MAK, Sarah S.T.

AU - LEUNG, Kenneth M.Y.

PY - 2017/11/30

Y1 - 2017/11/30

N2 - A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200 μg/L Cu, EC50 for the yield at 25 °C; and 1000 μg/L Cu, EC50 for growth inhibition at 25 °C) were conducted against four temperatures (10 °C, 15 °C, 25 °C and 30 °C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30 °C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90–2 and sit1 (related to silica shell formation) were highly expressed at 30 °C under 1000 μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15 °C under 200 μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.

AB - A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200 μg/L Cu, EC50 for the yield at 25 °C; and 1000 μg/L Cu, EC50 for growth inhibition at 25 °C) were conducted against four temperatures (10 °C, 15 °C, 25 °C and 30 °C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30 °C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90–2 and sit1 (related to silica shell formation) were highly expressed at 30 °C under 1000 μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15 °C under 200 μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.

KW - Copper

KW - Heat shock protein

KW - Marine diatom

KW - Photosynthesis

KW - Thalassiosira

KW - Thermal stress

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Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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