Phylogenetic Systematics of the Water Toad (Bufo stejnegeri) Elucidates the Evolution of Semi-aquatic Toad Ecology and Pleistocene Glacial Refugia

Jonathan J. FONG, Bao Tian YANG, Pi Peng LI, Bruce WALDMAN, Mi Sook MIN*

*Corresponding author for this work

Research output: Journal PublicationsJournal Article (refereed)

Abstract

The water toad (Bufo stejnegeri) is endemic to Northeastern Asia (South Korea, North Korea, and China) and has unique ecology for a toad by being semi-aquatic and breeding in lotic environments. We use a suite of phylogenetic analyses to understand the evolution of B. stejnegeri's distinctive ecology and the impact of Pleistocene glacial cycles on the biodiversity of Northeast Asia. For the evolution of a semi-aquatic lifestyle, although B. stejnegeri is relatively closely related to two other semi-aquatic Bufo species (B. torrenticola and B. andrewsi), ancestral state reconstruction analysis infers an independent evolution in all three species. Upon closer inspection, B. stejnegeri exhibits major differences in amplexus and egg-laying behavior compared to the other two species, supporting independent evolution. Divergence dating analyses infer B. stejnegeri to have originated during the Late Miocene-Early Pliocene (4.3 Ma, 2.7–6.2 Ma). This species does not exhibit population differentiation with respect to mountain range, but shows a preliminary genetic pattern of southern richness and northern purity supporting a single refugium in Korea during Pleistocene glacial cycles. The Bayesian skyline plot supports this inference, suggesting a population decline followed by expansion during the Pleistocene. Although not as species rich as the tropics, we hope this study helps spark interest in Northeast Asian biodiversity.

Original languageEnglish
Article number523
JournalFrontiers in Ecology and Evolution
Volume7
DOIs
Publication statusPublished - 22 Jan 2020

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Bufo
toad
toads
refugium
refuge habitats
Pleistocene
ecology
taxonomy
phylogenetics
phylogeny
water
North Korea
biodiversity
lotic systems
lotic environment
South Korea
purity
lifestyle
Korean Peninsula
population decline

Bibliographical note

Data Availability Statement
The datasets generated for this study can be found in GenBank with the following accession numbers: MK031411–MK031654.

Ethics Statement
The animal study was reviewed and approved by Seoul National University Ethics Committee.

Author Contributions
JF, B-TY, P-PL, BW, and M-SM designed the study and wrote the manuscript. JF, B-TY, and M-SM collected samples. JF and B-TY analyzed the data.

Funding
This research was supported by grants from the National Research Foundation of Korea (2012K1A2B1A03000496) funded by the Government of the Republic of Korea (MSIP), and the Research Institute of Basic Sciences, Seoul National University (to BW), and from the National Natural Science Foundation of China (31211140342) (to P-PL). We thank Profs. Wenbo Liao and Jinzhong Fu for information on the reproductive biology of B. andrewsi. We would like to thank members of the Min and Waldman labs for help with fieldwork, and thank Adam Leache for help with divergence dating analyses.

Keywords

  • ancestral state reconstruction
  • Baekdudaegan Mountain Range
  • Bayesian skyline
  • divergence dating
  • Korea
  • Northeast Asia

Cite this

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title = "Phylogenetic Systematics of the Water Toad (Bufo stejnegeri) Elucidates the Evolution of Semi-aquatic Toad Ecology and Pleistocene Glacial Refugia",
abstract = "The water toad (Bufo stejnegeri) is endemic to Northeastern Asia (South Korea, North Korea, and China) and has unique ecology for a toad by being semi-aquatic and breeding in lotic environments. We use a suite of phylogenetic analyses to understand the evolution of B. stejnegeri's distinctive ecology and the impact of Pleistocene glacial cycles on the biodiversity of Northeast Asia. For the evolution of a semi-aquatic lifestyle, although B. stejnegeri is relatively closely related to two other semi-aquatic Bufo species (B. torrenticola and B. andrewsi), ancestral state reconstruction analysis infers an independent evolution in all three species. Upon closer inspection, B. stejnegeri exhibits major differences in amplexus and egg-laying behavior compared to the other two species, supporting independent evolution. Divergence dating analyses infer B. stejnegeri to have originated during the Late Miocene-Early Pliocene (4.3 Ma, 2.7–6.2 Ma). This species does not exhibit population differentiation with respect to mountain range, but shows a preliminary genetic pattern of southern richness and northern purity supporting a single refugium in Korea during Pleistocene glacial cycles. The Bayesian skyline plot supports this inference, suggesting a population decline followed by expansion during the Pleistocene. Although not as species rich as the tropics, we hope this study helps spark interest in Northeast Asian biodiversity.",
keywords = "ancestral state reconstruction, Baekdudaegan Mountain Range, Bayesian skyline, divergence dating, Korea, Northeast Asia",
author = "FONG, {Jonathan J.} and YANG, {Bao Tian} and LI, {Pi Peng} and Bruce WALDMAN and MIN, {Mi Sook}",
note = "Data Availability Statement The datasets generated for this study can be found in GenBank with the following accession numbers: MK031411–MK031654. Ethics Statement The animal study was reviewed and approved by Seoul National University Ethics Committee. Author Contributions JF, B-TY, P-PL, BW, and M-SM designed the study and wrote the manuscript. JF, B-TY, and M-SM collected samples. JF and B-TY analyzed the data. Funding This research was supported by grants from the National Research Foundation of Korea (2012K1A2B1A03000496) funded by the Government of the Republic of Korea (MSIP), and the Research Institute of Basic Sciences, Seoul National University (to BW), and from the National Natural Science Foundation of China (31211140342) (to P-PL). We thank Profs. Wenbo Liao and Jinzhong Fu for information on the reproductive biology of B. andrewsi. We would like to thank members of the Min and Waldman labs for help with fieldwork, and thank Adam Leache for help with divergence dating analyses.",
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Phylogenetic Systematics of the Water Toad (Bufo stejnegeri) Elucidates the Evolution of Semi-aquatic Toad Ecology and Pleistocene Glacial Refugia. / FONG, Jonathan J.; YANG, Bao Tian; LI, Pi Peng; WALDMAN, Bruce; MIN, Mi Sook.

In: Frontiers in Ecology and Evolution, Vol. 7, 523, 22.01.2020.

Research output: Journal PublicationsJournal Article (refereed)

TY - JOUR

T1 - Phylogenetic Systematics of the Water Toad (Bufo stejnegeri) Elucidates the Evolution of Semi-aquatic Toad Ecology and Pleistocene Glacial Refugia

AU - FONG, Jonathan J.

AU - YANG, Bao Tian

AU - LI, Pi Peng

AU - WALDMAN, Bruce

AU - MIN, Mi Sook

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PY - 2020/1/22

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N2 - The water toad (Bufo stejnegeri) is endemic to Northeastern Asia (South Korea, North Korea, and China) and has unique ecology for a toad by being semi-aquatic and breeding in lotic environments. We use a suite of phylogenetic analyses to understand the evolution of B. stejnegeri's distinctive ecology and the impact of Pleistocene glacial cycles on the biodiversity of Northeast Asia. For the evolution of a semi-aquatic lifestyle, although B. stejnegeri is relatively closely related to two other semi-aquatic Bufo species (B. torrenticola and B. andrewsi), ancestral state reconstruction analysis infers an independent evolution in all three species. Upon closer inspection, B. stejnegeri exhibits major differences in amplexus and egg-laying behavior compared to the other two species, supporting independent evolution. Divergence dating analyses infer B. stejnegeri to have originated during the Late Miocene-Early Pliocene (4.3 Ma, 2.7–6.2 Ma). This species does not exhibit population differentiation with respect to mountain range, but shows a preliminary genetic pattern of southern richness and northern purity supporting a single refugium in Korea during Pleistocene glacial cycles. The Bayesian skyline plot supports this inference, suggesting a population decline followed by expansion during the Pleistocene. Although not as species rich as the tropics, we hope this study helps spark interest in Northeast Asian biodiversity.

AB - The water toad (Bufo stejnegeri) is endemic to Northeastern Asia (South Korea, North Korea, and China) and has unique ecology for a toad by being semi-aquatic and breeding in lotic environments. We use a suite of phylogenetic analyses to understand the evolution of B. stejnegeri's distinctive ecology and the impact of Pleistocene glacial cycles on the biodiversity of Northeast Asia. For the evolution of a semi-aquatic lifestyle, although B. stejnegeri is relatively closely related to two other semi-aquatic Bufo species (B. torrenticola and B. andrewsi), ancestral state reconstruction analysis infers an independent evolution in all three species. Upon closer inspection, B. stejnegeri exhibits major differences in amplexus and egg-laying behavior compared to the other two species, supporting independent evolution. Divergence dating analyses infer B. stejnegeri to have originated during the Late Miocene-Early Pliocene (4.3 Ma, 2.7–6.2 Ma). This species does not exhibit population differentiation with respect to mountain range, but shows a preliminary genetic pattern of southern richness and northern purity supporting a single refugium in Korea during Pleistocene glacial cycles. The Bayesian skyline plot supports this inference, suggesting a population decline followed by expansion during the Pleistocene. Although not as species rich as the tropics, we hope this study helps spark interest in Northeast Asian biodiversity.

KW - ancestral state reconstruction

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KW - Bayesian skyline

KW - divergence dating

KW - Korea

KW - Northeast Asia

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DO - 10.3389/fevo.2019.00523

M3 - Journal Article (refereed)

AN - SCOPUS:85079034076

VL - 7

JO - Frontiers in Ecology and Evolution

JF - Frontiers in Ecology and Evolution

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