Sequence validation for the identification of the white-rot fungi Bjerkandera in public sequence databases

Paul Eunil JUNG, Jonathan J. FONG, Myung Soo PARK, Seung Yoon OH, Changmu KIM, Young Woon LIM

Research output: Journal PublicationsJournal Article (refereed)

13 Citations (Scopus)

Abstract

White-rot fungi of the genus Bjerkandera are cosmopolitan and have shown potential for industrial application and bioremediation. When distinguishing morphological characters are no longer present (e.g., cultures or dried specimen fragments), characterizing true sequences of Bjerkandera is crucial for accurate identification and application of the species. To build a framework for molecular identification of Bjerkandera, we carefully identified specimens of B. adusta and B. fumosa from Korea based on morphological characters, followed by sequencing the internal transcribed spacer region and 28S nuclear ribosomal large subunit. The phylogenetic analysis of Korean Bjerkandera specimens showed clear genetic differentiation between the two species. Using this phylogeny as a framework, we examined the identification accuracy of sequences available in GenBank. Analyses revealed that many Bjerkandera sequences in the database are either misidentified or unidentified. This study provides robust reference sequences for sequence-based identification of Bjerkandera, and further demonstrates the presence and dangers of incorrect sequences in GenBank.
Original languageEnglish
Pages (from-to)1301-1307
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume24
Issue number10
Early online date9 Jun 2014
DOIs
Publication statusPublished - 1 Oct 2014
Externally publishedYes

Keywords

  • Sequence validation
  • ITS
  • 28S nuclear ribosomal large subunit
  • white-rot fungi
  • Bjerkandera
  • GenBank

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