Species delimitation of three species within the Russula subgenus Compacta in Korea : R. eccentrica, R. nigricans, and R. subnigricans

Myung Soo PARK, Hyun LEE, Seung Yoon OH, Paul Eunil JUNG, Soon Ja SEOK, Jonathan, Julio FONG, Young Woon LIM

Research output: Journal PublicationsJournal Article (refereed)Researchpeer-review

7 Citations (Scopus)

Abstract

Distinguishing individual Russula species can be very difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. We focus on two morphologically similar species that are often misidentified for each other: R. nigricans and R. subnigricans . Based on molecular phylogenetic analyses, we identify three subgroups of R. nigricans , with two from Asia and one from Europe/North America. Surprisingly, we find Korean R. subnigricans are more closely related to R. eccentrica from North America than the type specimen of R. subnigricans from Japan. These molecular data, along with habitat data, reveal that Korean R. subnigricans had previously been misclassified and should now be recognized as R. eccentrica . Both ITS and LSU exhibit high interspecific and low intraspecific variation for R. eccentrica , R. nigricans , and R. subnigricans . These markers provide enough resolutional power to differentiate these species and uncover phylogeographic structure, and will be powerful tools for future ecological studies of Russula .
Original languageEnglish
Pages (from-to)631-638
Number of pages8
JournalJournal of Microbiology
Volume52
Issue number8
Early online date4 Jul 2014
DOIs
Publication statusPublished - Aug 2014
Externally publishedYes

Fingerprint

Korea
North America
Large Ribosome Subunits
Ecosystem
Japan

Keywords

  • DNA barcoding
  • Internal Transcribed Spacer
  • 28S Nuclear Ribosomal Large Subunit
  • Russula eccentrica
  • Russula subnigricans
  • Russula nigricans

Cite this

PARK, Myung Soo ; LEE, Hyun ; OH, Seung Yoon ; JUNG, Paul Eunil ; SEOK, Soon Ja ; FONG, Jonathan, Julio ; LIM, Young Woon. / Species delimitation of three species within the Russula subgenus Compacta in Korea : R. eccentrica, R. nigricans, and R. subnigricans. In: Journal of Microbiology. 2014 ; Vol. 52, No. 8. pp. 631-638.
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abstract = "Distinguishing individual Russula species can be very difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. We focus on two morphologically similar species that are often misidentified for each other: R. nigricans and R. subnigricans . Based on molecular phylogenetic analyses, we identify three subgroups of R. nigricans , with two from Asia and one from Europe/North America. Surprisingly, we find Korean R. subnigricans are more closely related to R. eccentrica from North America than the type specimen of R. subnigricans from Japan. These molecular data, along with habitat data, reveal that Korean R. subnigricans had previously been misclassified and should now be recognized as R. eccentrica . Both ITS and LSU exhibit high interspecific and low intraspecific variation for R. eccentrica , R. nigricans , and R. subnigricans . These markers provide enough resolutional power to differentiate these species and uncover phylogeographic structure, and will be powerful tools for future ecological studies of Russula .",
keywords = "DNA barcoding, Internal Transcribed Spacer, 28S Nuclear Ribosomal Large Subunit, Russula eccentrica, Russula subnigricans, Russula nigricans",
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Species delimitation of three species within the Russula subgenus Compacta in Korea : R. eccentrica, R. nigricans, and R. subnigricans. / PARK, Myung Soo; LEE, Hyun; OH, Seung Yoon; JUNG, Paul Eunil; SEOK, Soon Ja; FONG, Jonathan, Julio; LIM, Young Woon.

In: Journal of Microbiology, Vol. 52, No. 8, 08.2014, p. 631-638.

Research output: Journal PublicationsJournal Article (refereed)Researchpeer-review

TY - JOUR

T1 - Species delimitation of three species within the Russula subgenus Compacta in Korea : R. eccentrica, R. nigricans, and R. subnigricans

AU - PARK, Myung Soo

AU - LEE, Hyun

AU - OH, Seung Yoon

AU - JUNG, Paul Eunil

AU - SEOK, Soon Ja

AU - FONG, Jonathan, Julio

AU - LIM, Young Woon

PY - 2014/8

Y1 - 2014/8

N2 - Distinguishing individual Russula species can be very difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. We focus on two morphologically similar species that are often misidentified for each other: R. nigricans and R. subnigricans . Based on molecular phylogenetic analyses, we identify three subgroups of R. nigricans , with two from Asia and one from Europe/North America. Surprisingly, we find Korean R. subnigricans are more closely related to R. eccentrica from North America than the type specimen of R. subnigricans from Japan. These molecular data, along with habitat data, reveal that Korean R. subnigricans had previously been misclassified and should now be recognized as R. eccentrica . Both ITS and LSU exhibit high interspecific and low intraspecific variation for R. eccentrica , R. nigricans , and R. subnigricans . These markers provide enough resolutional power to differentiate these species and uncover phylogeographic structure, and will be powerful tools for future ecological studies of Russula .

AB - Distinguishing individual Russula species can be very difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. We focus on two morphologically similar species that are often misidentified for each other: R. nigricans and R. subnigricans . Based on molecular phylogenetic analyses, we identify three subgroups of R. nigricans , with two from Asia and one from Europe/North America. Surprisingly, we find Korean R. subnigricans are more closely related to R. eccentrica from North America than the type specimen of R. subnigricans from Japan. These molecular data, along with habitat data, reveal that Korean R. subnigricans had previously been misclassified and should now be recognized as R. eccentrica . Both ITS and LSU exhibit high interspecific and low intraspecific variation for R. eccentrica , R. nigricans , and R. subnigricans . These markers provide enough resolutional power to differentiate these species and uncover phylogeographic structure, and will be powerful tools for future ecological studies of Russula .

KW - DNA barcoding

KW - Internal Transcribed Spacer

KW - 28S Nuclear Ribosomal Large Subunit

KW - Russula eccentrica

KW - Russula subnigricans

KW - Russula nigricans

UR - http://commons.ln.edu.hk/sw_master/5037

U2 - 10.1007/s12275-014-4168-z

DO - 10.1007/s12275-014-4168-z

M3 - Journal Article (refereed)

VL - 52

SP - 631

EP - 638

JO - Journal of Microbiology

JF - Journal of Microbiology

SN - 1225-8873

IS - 8

ER -